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质谱技术信息速递14-07-11

 
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2014年07月11日

WHAT'S NEW WITH DART?

The latest developments and news on DART MS

华质泰科 DART 质谱资讯速递

为您传递实时直接分析质谱最新进展和信息

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尊敬的老师:

不论您是否参加了美国巴尔地摩的美国质谱年会(ASMS2014),我们都很乐意和您一起分享并回顾近期的一些精彩讲座及报告。请点击我们提供的链接来浏览报告内容和ASMS 用户会 DART 技术论坛内容,此链接涵盖了八位演讲人提供的最新 DART-MS 研究成果,同时,我们附上 ASMS2014 年会期间演讲及墙报展示的与 DART 及关的文章或摘要,精彩纷呈,不容错过哦!

We enjoyed meeting many of you at the ASMS meeting in Baltimore and want to provide you with the most recent presentations. For those of you that did not attend, we have links here to the presentations as well as the DART Forum where 8 presenters showcased their latest research using DART-MS.

Feel free to contact me if you have any questions or comments. Thanks. 

Regards,

您有任何疑问或建议欢迎联系我们 。谢谢并顺祝商祺!

贾万银  市场部
华质泰科生物技术(北京)有限公司
ASPEC Technologies Limited

北京市朝阳区安立路60号院润枫德尚A座1506室,邮编:100102
电话:+86-10-6439-9978
传真:+86-10-6439-9499
电子邮箱:info@aspectechnologies.com
网址:www.aspectechnologies.com

如果无法看到邮件内容,请您点击这里

其它精彩内容:AIMS官方网站  DART专题

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Included in this newsletter:
本则速递中包含有:

 

Recent Presentations from ASMS 2014
ASMS2014 DART 用户会技术论坛专题及演讲报告

Recent DART Publications
近期 DART 出版物

 
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Recent DART Publications
近期 DART 出版物

 

Accurate Mass Fragment Library for Rapid Analysis of Pesticides on Produce Using Ambient Pressure Desorption Ionization with High-Resolution Mass Spectrometry
使用原位电离 DART-MS 高分辨质谱技术并对照精确质量片段库来快速分析农产品中的农药

Sara E. Kern, Lora A. Lin, Frederick L. Fricke
U.S. Food and Drug Administration, Forensic Chemistry Center, 6751 Steger Drive, Cincinnati, OH, 45237, USA

J.AM. Soc. Mass Spectrom., pp. 1-7, May 2014

Abstract:

U.S. food imports have been increasing steadily for decades, intensifying the need for a rapid and sensitive screening technique. A method has been developed that uses foam disks to sample the surface of incoming produce. This work provides complimentary information to the extensive amount of published pesticide fragmentation data collected using LCMS systems (Sack et al. Journal of Agricultural and Food Chemistry, 59, 6383-6411, 2011; Mol et al. Analytical and Bioanalytical Chemistry, 403, 2891-2908, 2012). The disks are directly analyzed using transmission-mode direct analysis in real time (DART) ambient pressure desorption ionization coupled to a high resolution accurate mass-mass spectrometer (HRAM-MS). In order to provide more certainty in the identification of the pesticides detected, a library of accurate mass fragments and isotopes of the protonated parent molecular ion (the [M+H]+) has been developed. The HRAM-MS is equipped with a quadrupole mass filter, providing the capability of "data-dependent" fragmentation, as opposed to "all -ion" fragmentation (where all of the ions enter a collision chamber and are fragmented at once). A temperature gradient for the DART helium stream and multiple collision energies were employed to detect and fragment 164 pesticides of varying chemical classes, sizes, and polarities. The accurate mass information of precursor ([M+H]+ ion) and fragment ions is essential in correctly identifying chemical contaminants on the surface of imported produce. Additionally, the inclusion of isotopes of the [M+H]+ in the database adds another metric to the confirmation process. The fragmentation data were collected using a Q-Exactive mass spectrometer and were added to a database used to process data collected with an Exactive mass spectrometer, an instrument that is more readily available for this screening application. The commodities investigated range from smooth-skinned produce such as apples to rougher surfaces like broccoli. The minimal sample preparation and absence of chromatography has shortened the analysis time to about 15 min per sample, and the simplicity and robustness of the technique make it ideal for rapid screening.

 

摘要:

近数十年来美国国内进口食品的数量呈逐年稳步增长的趋势,相对应地,对于能用于快速灵敏地筛查与确认的分析技术的渴求也愈来愈强烈。我们报告一种利用泡沫(海绵)片对入口农产品进行表面采样并随即利用 DART-MS 快速分析和确证的方法。该方法提供了和已发表的含有大量的以 LCMS 系统收集到的农药质谱裂解碎片数据(Sack et al. Journal of Agricultural and Food Chemistry, 59, 6383-6411, 2011; Mol et al. Analytical and Bioanalytical Chemistry, 403, 2891-2908, 2012)互为补充的结果。泡沫(海绵)片直接以透过式模块载样输送至 DART-高分辨质谱仪进行直接分析。为了更确切地鉴定农药,我们建立了质子化母离子 [M+H]+ 的精确质量和同位素信息数据库。该 HRAM(高分辨、高质量精度)质谱配备四极杆质量过滤器,拥有“数据敲定(data-dependent)”的碰撞活化裂解能力。该能力 和“所有离子all -ion”裂解(所有的离子进入碰撞室后同时裂解)的质谱法有所不同。利用 DART 氦气的温度梯度及质谱仪的多种碰撞活化能量,可以检测并裂解164种不同种类、不同大小和不同极性的化学农药。前体母离子和碎片子离子的精确质量信息对于识别进口农产品表面化学污染物至关重要。此外,数据库中的母离子的同位素信息则提供了另一个衡量标准来进一步辅助识别。碎片数据的收集通过 Q-Exactive 型号的质谱来完成,之后添加到早先采用 Exactive 型号的质谱仪收集建立的数据库当中。后者Exactive为较低端的型号,在普通筛查实验室更容易配置。商品筛查的范围非常广泛,既有果皮光滑的产品如苹果,又有表面粗糙的品种如花菜。极少量或者几乎不需要的样品制备、和无需色谱分离的特性,使该技术将每个样品的分析周期大大缩短到了只有15分钟,其简单、稳定和实用的特性非常适用于快速筛查的目的。

 

Analytical strategies for controlling polysorbate-based nanomicelles in fruit juice
控制果汁中山梨醇纳米胶束的分析策略

Veronika Krtkova, Vera Schulzova, Ondrej Lacina, Vojtech Hrbek, Monika Tomaniova, Jana Hajslova ICT Prague, Technicka 3, 166 28, Prague, Czech Republic

Abstract:

This study focused on the detection and quantification of organic micelle-type nanoparticles (NPs) with polysorbate components (polysorbate 20 and polysorbate 80) in their micelle shells that could be used to load biologically active compounds into fruit juice. Several advanced analytical techniques were applied in the stepwise method development strategy used. In the first phase, a system consisting of ultrahigh-performance liquid chromatography employing a size exclusion column coupled with an evaporative light scattering detector (UHPLC-SEC-ELSD) was used for the fractionation of micelle assemblies from other, lower molecular weight sample components. The limit of detection (LoD) of these polysorbate micelles in spiked apple juice was 500 μg mL−1. After this screening step, mass spectrometric (MS) detection was utilized to confirm the presence of polysorbates in the detected micelles. Two alternative MS techniques were tested: (i) ambient high-resolution mass spectrometry employing a direct analysis in real time ion source coupled with an Orbitrap MS analyzer (DART-Orbitrap MS) enabled fast and simple detection of the polysorbates present in the samples, with a lowest calibration level (LCL) of 1000 μg mL−1; (ii) ultrahigh-performance reversed-phase liquid chromatography coupled with high-resolution time-of-flight mass spectrometry (UHPLC-HRTOF-MS) provided highly selective and sensitive detection and quantification of polysorbates with an LCL of 0.5 μg mL−1.

 

摘要:

本文的研究重点是定量检测含有山梨醇成分(聚山梨醇酯-80和聚山梨醇酯-20)的有机胶束纳米粒子,这种胶束纳米粒子可负载果汁中具有生物活性的化合物成分。几种先进的分析技术根据分段发展策略被应用于检测。在第一阶段中,由 UHPLC(超高效液相色谱)、SEC(分子排阻柱)及 ELSD(蒸汽光散射检测器)组成的系统用于胶束和其他低分子量成分的分离。此方法对加入标准的苹果汁中山梨醇微胶粒的检测限(LOD)为500μg ml−1。此此筛选步骤后,质谱(MS)检测被用来进一步确定微胶粒中山梨醇的存在。我们测试了两种可供选择的质谱技术:(1)实时直接分析-高分辨率质谱系统,即 DART- Orbitrap 质谱,实现样品中山梨醇的快速简便检测;(2)UHPLC-HRTOF-MS 具有高选择性和高灵敏度的特性,能够在最低校准水平0.5μg的情况下定量山梨醇。

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About ASPEC

关于华质泰科

华质泰科生物技术(北京)有限公司(简称“华质泰科”或“ASPEC”)致力于引领行业领域中的实时科学发展潮流,通过专业的技术、成熟的产品和持续高品质的服务为客户赢得更多、更好的投资回报。华质泰科是美国 IonSense 公司 DART®  实时直接分析质谱离子源、ASAP 大气压固体分析探针、美国 Advion 公司 LESA-enabled TriVersa NanoMate 芯片多通道纳喷离子源、瑞士ColvaX 公司 HM3 超高分子量 MALDI 质谱检测系统、美国 PMI 公司 Byonic 蛋白质组学质谱软件、美国 Phoenix S&T 公司双柱-双纳喷离子源及纳升柱控温盒、加拿大 Phytronix LDTD 激光热解析离子源等技术以及产品在大中华地区的总代理商。

 

ASPEC develops solutions to help advance innovations in real-time science and technology. We support scientists in chemical and life science industries by offering premier bio-analytical instruments, consulting and services. We are conducting sales, marketing, and support functions as an exclusive distributor of IonSense, Advion, CovalX, Phytronix, and many others in the Greater China region. ASPEC founded and co-organized the Ambient Ionization Mass Spectrometry Conference China, since 2013.

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About IonSense

 

IonSense, Inc. provides OpenSpot Mass Spectrometry™ solutions to the fields of food safety, forensics, drug development, and chemical analysis. We manufacture and develop direct analysis in real time (DART®) technology licensed from JEOL USA, Inc. and atmospheric solids analysis probe (ASAP™) licensed from M&M Consulting.

 

DART and ASAP Sources are available for most commercial LC/MS systems. Look here to see if your system is DART-ready. And check here to see if your system is ASAP-ready.

 
 
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