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华质泰科-质谱资讯速递
为您传递DART®, LESA等实时直接分析质谱最新进展和信息

 

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近期 DART 出版物

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  Rapid quality assessment of Radix Aconiti Preparata using direct analysis in real time mass spectrometry
  实时直接分析质谱（DART）在制川乌快速质量控制中的应用
   

  Hongbin Zhu, Chunyan Wang, Yao Qi, Fengrui Song, Zhiqiang Liu, Shuying Liu 
  Changchun Center of Mass Spectrometry & Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  University of Chinese Academy of Sciences, Beijing 100039, China

  朱洪彬1,2，刘 舒1,2，宋凤瑞1*，刘志强1，刘淑莹1 
  （ 1. 中国科学院长春应用化学研究所，长春质谱中心，吉林 长春 130022； 2. 中国科学院研究生部，北京 100049）

  This study presents a novel and rapid method to identify chemical markers for the quality control of Radix Aconiti Preparata, a world widely used traditional herbal medicine. In the method, the samples with a fast extraction procedure were analyzed using direct analysis in real time mass spectrometry (DART MS) combined with multivariate data analysis. At present, the quality assessment approach of Radix Aconiti Preparata was based on the two processing methods recorded in Chinese Pharmacopoeia for the purpose of reducing the toxicity of Radix Aconiti and ensuring its clinical therapeutic efficacy. In order to ensure the safety and effectivity in clinical use, the processing degree of Radix Aconiti should be well controlled and assessed. In the paper, hierarchical cluster analysis and principal component analysis were performed to evaluate the DART MS data of Radix Aconiti Preparata samples in different processing times. The results showed that the well processed Radix Aconiti Preparata, unqualified processed and the raw Radix Aconiti could be clustered reasonably corresponding to their constituents. The loading plot shows that the main chemical markers having the most influence on the discrimination amongst the qualified and unqualified samples were mainly some monoester diterpenoid aconitines and diester diterpenoid aconitines, i.e. benzoylmesaconine, hypaconitine, mesaconitine, neoline, benzoyl hypaconine, benzoylaconine, fuziline, aconitine and 10-OH-mesaconitine. The established DART MS approach in combination with multivariate data analysis provides a very flexible and reliable method for quality assessment of toxic herbal medicine.

  由于制川乌的炮制程度直接关系到其在临床应用中的安全、有效，因此，控制、检测其炮制程度是制川乌质量标准研究的重要方面。本研究采用新型的质谱离子化技术，对中国药典中制川乌的快速质量控制方法进行了研究。经快速简单处理后的样品直接用于实时直接分析质谱（DART MS）分析，并结合化学计量学方法对其 DART MS 指纹图谱进行评价。通过系统聚类分析（HCA）和主成分分析（PCA）对不同炮制时间制川乌的 DART MS指纹图谱的评价，确定了制川乌质量控制的化学标志性物质。结果表明，通过 DART MS 结合化学计量学分析能够对合格的制川乌、不合格制川乌以及生川乌进行鉴别。主成分分析载荷图结果表明，制川乌质量控制的主要化学标志物为苯甲酰新乌头原碱，次乌头碱，新乌头碱，尼奥灵，苯甲酰次乌头原碱，苯甲酰乌头原碱，附子灵，乌头碱和 10-OH-新乌头碱。本研究证明实时直接分析质谱技术（DART MS）快速可靠，直接通过谱图分析或结合化学计量学方法可以应用于制川乌的快速质量控制。

   

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  Evaluating agarwood products for 2-(2-phenylethyl)chromones using direct analysis in real time time-of-flight mass spectrometry
  DART 飞行时间质谱鉴定沉香木制品的 2-（2-苯乙基）色酮化合物

  Cady Lancaster, Edgard Espinoza
  National Fish & Wildlife Forensic Lab, Ashland, OR, USA 
  
  RATIONALE
  原理阐述
  
  Agarwood is the resinous material harvested from threatened Aquilaria species. We investigated how many protonated 2-(2-phenylethyl)chromone ions were sufficient to make an accurate identification of agarwood. Analysis of 125 reference samples was carried out by direct analysis in real time time-of-flight mass spectrometry (DART-TOFMS). The identification criteria developed were applied to commercial samples.
  
  沉香木是一种树脂性材料，来源于濒临灭绝的沉香种类。我们研究了多个质子化的 2 -（2-苯乙基）色酮离子能足够用来准确地鉴定沉香木。利用 DART 飞行时间质谱分析了近 125 份参考样本，以便将所发现的鉴定标准应用于流通商品的筛选分析。
  
  METHODS
  方法
  
  We developed a technique that uses DART-TOFMS to detect 2-(2-phenylethyl)chromones. Additionally, we developed a set of criteria to infer the presence of Aquilaria in commercial samples of wood chips, sawdust, incense and liquids. Additionally, we examined other fragrant woods to determine if they contained a chemical profile that could be falsely identified as agarwood.
  
  我们开发了使用 DART-TOFMS 来检测 2-（2-苯乙基）色酮化合物的技术，创建了一套标准来推断流通商品（如木片、木屑、香及液体）中沉香木是否存在，检查了其他香木来确定他们是否含有易被误判为沉香木的化学图谱。
  
  RESULTS
  结果
  
  Analysis of reference and commercial samples (n = 151) established that DART-TOFMS provides reproducible mass spectra that are useful for inferring the genus of suspected agarwood samples. We identified 17 ions which were useful for authenticating agarwood. Comparison of the number of chromone ions detected by direct analyses of dry wood chips versus eluent analysis of methanol-extracted wood showed that results were similar. Lastly, analysis of 25 scented woods of other species did not give false positive results.
  
  所建立的标准样本及商业样本（n=151）的 DART-TOFMS 分析方法，提供了重现的、能有效地推断疑似沉香木样本种类的质谱图。其中的 17 种离子可都被用于验证沉香木的真假。对甲醇萃取液和对干木屑直接分析显示了相似数目的色酮离子。而其他 25 种有香味的木头类均没有得出误检结果。
  
  CONCLUSIONS
  结论
  
  Reliable criteria for inferring agarwood include the presence of diagnostic ions, m/z 319.118 or 349.129, in addition to ten or more ions characteristic of 2-(2-phenylethyl)chromones. Wood anatomists challenged with difficult morphological identifications can use this tool to assist in their analyses. 
  
  推断沉香木的可靠标准除了十几种特征的 2-（2-苯乙基）色酮离子还包括 m/z 319.118 及3 49.129 诊断离子的存在与否。当木质剖析者遇到很难从形态上鉴别的情况时可以用这个方法来协助分析。

  详情请点击这里
  
  

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关于 IonSense

IonSense, Inc. provides OpenSpot™ Mass Spectrometry solutions to the fields of food safety, forensics, drug development, and chemical analysis. They manufacture and develop direct analysis in real time ( DART® ) technology licensed from JEOL USA, Inc. and atmospheric solids analysis probe ( ASAP™ ) licensed from M&M Consulting. DART and ASAP Sources are available for most commercial LC/MS systems.  Look here to see if your system is DART-ready.  And check here to see if your system is ASAP-ready.

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关于华质泰科
 

华质泰科生物技术（北京）有限公司（简称“华质泰科”或“ASPEC”）致力于引领行业领域中的实时科学发展潮流，通过专业的技术、成熟的产品和持续高品质的服务为客户赢得更多、更好的投资回报。华质泰科是美国 IonSense公司 DART^® 实时直接分析离子源、ASAP 大气压固体分析探针、美国 Advion 公司 LESA 暨 TriVersa NanoMate 芯片多通道纳喷离子源、加拿大 BSI 公司 PEAKS 蛋白质组学质谱软件、美国 Phoenix S&T 公司双柱-双纳喷离子源、加拿大 Phytronix LDTD 激光热解析离子源等技术以及产品在大中华地区的总代理商。