华质泰科-质谱资讯速递
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近期 LESA 出版物

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  Screening of antibiotics from bacterial surfaces using LESA
  LESA 质谱高通量筛查细菌表面的抗生素
   

  The mass spectrometry MS research group of the Max Plank Institute for Chemical Ecology along with MEDINA and Merck Inc. have published their work demonstrating the TriVersa NanoMate with LESA capability for the fast and cost-effective screening of antibiotics from bacteria cultures in agar plates. Their paper published in Rapid Communications in Mass Spectrometry earlier this year shows，as following:
  
  马克斯普朗克研究所化学生态学质谱研究小组和 MEDINA 及默克公司已经发表了他们的工作成果，证明了 TVNM 和 LESA 能够快速及经济有效地从琼脂皿培养细菌中筛选抗生素，他们的文章发表在今年年初的 RCM 上，内容如下：
  
  RATIONALE 背景：
  
  There is a need to find new antibiotic agents to fight resistant pathogenic bacteria. To search successfully for novel antibiotics from bacteria cultivated under diverse conditions, we need a fast and cost-effective screening method.
  研发新的抗生素来对抗耐药菌刻不容缓。我们需要一种快速、经济的筛选方法，以便自多种培养条件下的大量细菌中成功地寻找新型抗生素。
  
  METHODS 方法:
  
  A combination of Liquid Extraction Surface Analysis (LESA), automated chip-based nanoelectrospray ionization, and high-resolution mass or tandem mass spectrometry using an Orbitrap XL was tested as the screening platform. Actinobacteria, known to produce well-recognized thiazolyl peptide antibiotics, were cultivated on a plate of solid medium and the antibiotics were extracted by organic solvent mixtures from the surface of colonies grown on the plate and analyzed using mass spectrometry (MS).
  液滴萃取表面分析技术（LESA）结合基于芯片的自动化纳升电喷雾离子化和高分辨质谱或串联质谱 Orbitrap XL被选为筛选平台。放线菌以产生易识别的噻唑肽类抗生素为众人所熟悉，我们把它放置在培养皿中培养，采用混合有机溶液萃取培养好后的细菌簇表面并进行质谱分析。
  
  RESULTS 结果:
  
  LESA combined with high-resolution MS is a powerful tool with which to extract and detect thiazolyl peptide antibiotics from different Actinobacteria. Known antibiotics were correctly detected with high mass accuracy (<4 ppm) and structurally characterized using tandem mass spectra. Our method is the first step toward the development of a novel high-throughput extraction and identification tool for antibiotics in particular and natural products in general.
  LESA 结合高分辨质谱在萃取及检测不同放线菌产生的噻唑肽类抗生素上展示出强大的优势，通过高质量精度（小于4 ppm），我们可以准确检测出已知抗生素，并根据串联质谱图进行结构确认。我们的方法是为设计针对天然产物开发尤其对特殊抗生素的高通量萃取和鉴定工具而迈出的第一步
  
  CONCLUSIONS 结论:
  
  The method described in this paper is suitable for (1) screening the natural products produced by bacterial colonies on cultivation plates within the first 2 min following extraction and (2) detecting antibiotics at high mass accuracy; the cost is around 2 Euro per sample.
  本文所描述的方法非常适合（1）快速检测在培养皿上培养的细菌菌群产生的天然产物，每个样品在萃取完后两分钟内完成检测，和（2）利用高质量精度来检测抗生素；每个样本检测成本约为 2 欧元。
  
  详情请点击这里
   

   

  Metabolomic studies from dried blood spots within the newborn screening program
  质谱代谢组学法筛查新生儿代谢缺陷症

  
  ​,Abstract 摘要 LESA

  
  A novel, single stage high resolution mass spectrometry-based method is presented for the population level screening of inborn errors of metabolism. The approach proposed here extends traditional electrospray tandem mass spectrometry screening by introducing nanospray ionization and high resolution mass spectrometry, allowing the selective detection of more than 400 individual metabolic constituents of blood including acylcarnitines, amino acids, organic acids, fatty acids, carbohydrates, bile acids, and complex lipids. Dried blood spots were extracted using a methanolic solution of isotope labeled internal standards, and filtered extracts were electrosprayed using a fully automated chip-based nanospray ion source in both positive and negative ion mode. Ions were analyzed using an Orbitrap Fourier transformation mass spectrometer at nominal mass resolution of 100 000. Individual metabolic constituents were quantified using standard isotope dilution methods. Concentration threshold (cutoff) level-based analysis allows the identification of newborns with metabolic diseases, similarly to the traditional electrospray tandem mass spectrometry (ESI-MS/MS) method; however, the detection of additional known biomarkers (e.g., organic acids) results in improved sensitivity and selectivity. The broad range of detected analytes allowed the untargeted multivariate statistical analysis of spectra and identification of additional diseased states, therapeutic artifacts, and damaged samples, besides the metabolic disease panel.
  
  本文介绍了一种新的单级高分辨质谱技术用于新生儿先天性代谢缺陷人群的筛查方法。该方法通过引入纳喷离子化及高分辨质谱技术来扩展传统电喷雾串联质谱在先天性代谢缺陷筛查方面的应用，它可以选择性检测超过400个不同的代谢产物，包括酰肉碱，氨基酸，有机酸，脂肪酸，碳水化合物，胆汁酸和复杂的脂类。采用含有同位素标记的内标的甲醇溶液对干血斑进行萃取，萃取液经过滤并通过全自动的芯片纳喷离子源纳喷，进行正、负离子模式分析。本文试验中采用了质量分辨率为十万（100,000）的傅里叶变换轨道阱质谱仪 Orbitrap 进行分析，并通过同位素稀释质谱法对不同的代谢成分进行定量分析。相似于传统电喷雾串联质谱法，基于浓度阈值（临界）水平的分析可以鉴定新生儿代谢疾病。然而，对于一些已知生物标记物（如有机酸），该方法表现出了更好的灵敏度和选择性。鉴于其对宽范围多组分的检测特征，该方法不仅可以用于代谢疾病筛查，还可以用于非特异性的谱图多元统计分析和对发生额外病变、人为治疗偏差、及受损样本的鉴定。
  
  详情请点击这里
  
  Analysis of chloroquine and metabolites directly from whole-body animal tissue sections by LESA
  LESA质谱研究动物组织中药物成像
  
  Abstract 摘要

  
  The rapid and direct analysis of the amount and spatial distribution of exogenous chloroquine (CHQ) and CHQ metabolites from tissue sections by liquid extraction surface sampling analysis coupled with tandem mass spectrometry (LESA-MS/MS) was demonstrated. LESA-MS/MS results compared well with previously published CHQ quantification data collected by organ excision, extraction and fluorescent detection. The ability to directly sample and analyze spatially resolved exogenous molecules from tissue sections with minimal sample preparation and analytical method development has the potential to facilitate the assessment of target tissue penetration of pharmaceutical compounds, to establish pharmacokinetic/pharmacodynamic relationships, and to complement established pharmacokinetic methods used in the drug discovery process during tissue distribution assessment.
  
  通过液滴萃取表面分析（LESA），外加串联质谱法（MS/MS），从组织切片中快速直接分析外源性氯喹及氯喹代谢物的含量及空间分布的做法早已得到证明。LESA-MS/MS（液滴萃取表面分析-串联质谱法）的结果比起先前发表的外源性氯喹定量结果 - 源自器官切除、提取及荧光检测等的繁琐流程 - 毫不逊色。LESA-MS/MS 直接取样分析外源性药物的空间分布对研究药物在目标组织中的渗透、研究建立药代动力学/药效关系、和在组织分布与评估过程中补充已建立的应用于药物发现过程药中的药代动力学方法等方面，具有潜力。
  
  详情请点击这里

  
  

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About Advion

Advion is a leader in ultra-high performance, small footprint mass spectroscopy & synthesis solutions for life science. We have unique and patented nanotechnologies, which deliver speed, flexibility, ease-of use, reliability and exceptional data quality. The TriVersa NanoMate and its nano-ESI Chip generate higher quality information from complex samples.

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关于华质泰科
 

华质泰科生物技术（北京）有限公司（简称“华质泰科”或“ASPEC”）致力于引领行业领域中的实时科学发展潮流，通过专业的技术、成熟的产品和持续高品质的服务为客户赢得更多、更好的投资回报。华质泰科是美国 IonSense 公司 DART® 实时直接分析质谱离子源、ASAP 大气压固体分析探针、美国 Advion 公司 LESA-enabled TriVersa NanoMate 芯片多通道纳喷离子源、加拿大 BSI 公司 PEAKS 蛋白质组学质谱软件、美国 Phoenix S&T 公司双柱-双纳喷离子源、加拿大 Phytronix LDTD 激光热解析离子源等技术以及产品在大中华地区的总代理商。

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