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质谱技术信息速递07

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华质泰科-质谱资讯速递
为您传递DART®, LESA等实时直接分析质谱最新进展和信息
 

 
尊敬的各位老师、用户:

当 DART GSX 渐渐频繁地出现在行业关键词当中时,请不要惊奇。近期实验发现,DART GSX 与 Cerno BioScience MassWorks™ 的结合应用使得其定性功能更加精确,详细内容请参阅本期速递。本期同时收录了几篇有关肝素钠、爆炸物及牛奶分析与检测的热点论文,以飧读者。

您有任何疑问或建议欢迎联系我们。谢谢并顺祝商祺!

贾万银  市场部
华质泰科生物技术(北京)有限公司
北京市朝阳区望京北路9号叶青大厦C座209室,邮编: 100102
电话:+86-10-6439-9978
传真: +86-10-6439-9499
电子邮箱:info@aspectechnologies.com
网址:www.aspectechnologies.com

DART® 专题
如果无法看到邮件内容,请您点击这里
 

 
  • Accurate Mass DART on a Quadrupole MS
    精确质量DART分析在四级杆质谱中的应用


    The DART GSX System, which is based on the Agilent GC/MSD, can rapidly screen for unwanted materials such as pesticides or phthalates in a matter of seconds.  However, there are times when higher specificity is needed, beyond the nominal mass accuracy provided by the MSD analyzer.

    DART GSX 系统,是基于 Agilent GC/MSD 基础之上的一种技术,几秒钟快速筛查残留或添加化合物,比如农残、邻苯二甲酸酯等塑化剂。但是,鉴定往往需要更高的特异性,远远超出常规的 MSD 质量分析器所能直接获得的质量精确度。

    Using the MassWorks Software, provided by Cerno Biosciences, this data can be converted to accuracies of a few milliDaltons, greatly enhancing specificity and aiding in identification.  Calibrating the spectra is simple and fast. The user loads the file containing the calibration mix (PFTBA) and a list of standard ions, then adjusts the peak selection to include isotope patterns. From this point the user could calibrate with the default parameters or adjust a few parameters if necessary.

    由Cerno Biosciences 提供的 MassWorks 软件,处理数据时分子量能精确到几个毫道尔顿,很大程度上加强了定性的能力。校正质谱简单快速;使用者仅需加载一个含有校正液(PFTBA,全氟三丁胺)系列标准离子的文件,然后选择一些离子包括同位素峰。使用者如是便可用默认参数或者有必要的话再调节一些参数来进行质量校正。

    This system and software were utilized for identifying pesticides and phthalates in herbal supplements.   A methanol extract of a ground Black Cohosh herbal supplement was used for this study. Four samples were each spiked with 1 PPM of the following phthalates or pesticides, DPP, BBP, Methamidophos and Acephate. The samples were then analyzed on the DART GSX at 250C using foam swabs dipped in the solution. The analysis of the 4 analytes was very quick, and the total run time including the calibrant was 3.5 minutes. After the calibration was run the values were recorded and the results are shown below.

    该系统和软件可以被用来鉴定在中草药补品中的农残和邻苯二甲酸盐酯类塑化剂。以甲醇萃取黑升麻草药。在四个样品中分别添加1ppm的邻苯二甲酸酯或农药:DPP、BBP、甲胺磷、乙酰甲胺磷,在250℃条件下用泡沫棒蘸取溶液直接在 DART GSX 上进行分析。包括仪器校正在内,样品的分析时间仅用了3.5min。校正之后数据被记录下来,结果如下:

    Sample 样品

    Nominal Mass   分子量

    Exact Mass绝对质量

    Accurate Mass精确质量

    mDA

    Methamidophos (甲胺磷)

    142.0

    142.0091

    142.0138

    4.6

    Acephate (乙酰甲胺磷)

    184.0

    184.0197

    184.0122

    7.5

    DiButyl Phthalate (邻苯二甲酸二丁酯)

    279.1

    279.1596

    279.1545

    5.1

    Benzyl Butyl Phthalte (邻苯二甲酸丁苄酯)

    313.1

    313.1439

    313.1305

    13.4

    With the increase in mass accuracy, the confidence is greatly increased in specifying the presence of a contaminant.  In addition an unknown compound may sometimes be identified due to its match in a formula search.

    随着质量准确度的增加,在详细区分出现的污染物时置信水平也在很大程度上得到提高。若再辅以分子式匹配搜索,未知物甚至能被轻松鉴定。

    \

    The DART GSX System can be fitted to one of your existing Agilent GC/MSD instruments or it can be supplied as a complete system with a new or refurbished Agilent GC/MSD.  

    DART GSX 系统能安装在您已有的 Agilent GC/MSD 仪器上,或作为一个完新的系统、或安装在翻新的 Agilent GC/MSD 系统上。

    For the details on the GSX, you can download the brochure here
    GSX 详情,请点击这里下载手册


 
Recent DART Publications
近期 LESA 出版物

  •  
    High-Throughput Differentiation of Heparin from Other Glycosaminoglycans by Pyrolysis Mass Spectrometry
    裂解质谱法从其他糖胺聚糖中高通量区分肝素钠

     
    Sensors with high chemical specificity and enhanced sample throughput are vital to screening food products and medical devices for chemical or biochemical contaminants that may pose a threat to public health. For example, the rapid detection of oversulfated chondroitin sulfate (OSCS) in heparin could prevent reoccurrence of heparin adulteration that caused hundreds of severe adverse events including deaths worldwide in 2007-2008. Here, rapid pyrolysis is integrated with direct analysis in real time (DART) mass spectrometry to rapidly screen major glycosaminoglycans, including heparin, chondroitin sulfate A, dermatan sulfate, and OSCS. The results demonstrate that, compared to traditional liquid chromatography-based analyses, pyrolysis mass spectrometry achieved at least 250-fold higher sample throughput and was compatible with samples volume-limited to about 300 nL. Pyrolysis yielded an abundance of fragment ions (e.g., 150 different m/z species), many of which were specific to the parent compound. Using multivariate and statistical data analysis models, these data enabled facile differentiation of the glycosaminoglycans with high throughput. After method development was completed, authentically contaminated samples obtained during the heparin crisis by the FDA were analyzed in a blinded manner for OSCS contamination. The lower limit of differentiation and detection were 0.1% (w/w) OSCS in heparin and 100 ng/μL (20 ng) OSCS in water, respectively. For quantitative purposes the linear dynamic range spanned approximately 3 orders of magnitude. Moreover, this chemical readout was successfully employed to find clues in the manufacturing history of the heparin samples that can be used for surveillance purposes. The presented technology and data analysis protocols are anticipated to be readily adaptable to other chemical and biochemical agents and volume-limited samples.

    高特异性、高灵敏度和高通量筛查食品及医疗设备中有害的化学或生物化学污染物对于保障公共安全和卫生至关重要。例如,在肝素中快速检测多硫软骨素(OSCS)能够阻止肝素掺假事故频发,肝素掺假导致严重的不良后果包括在2007-2008年全世界范围内的严重的死亡事件。快速裂解结合实时直接分析质谱(DART-MS)可以快速筛查几种主要的糖胺聚糖成分,包括肝素、硫酸软骨素A、硫酸皮肤素、和多硫软骨素。与基于传统的液质方法相比,裂解质谱法通量至少可以提高250倍,并且与有限的样品体积如300nL相兼容。裂解产生大量的裂解产物离子(例如,150种不同的m/z),其中很多是特征的母离子。借助多变量统计分析数据模型,能轻易、高通量区分糖胺聚糖。据此方法,盲样抽查了美国 FDA 缴获的许多被多硫软骨素污染的肝素纳样品。识别下线和检出限分别是在肝素中含有0.1%(w/w)多硫软骨素,和水溶液中100ng/uL(20ng)多硫软骨素。定量分析线性动态范围跨越达3个数量级。此外,这一化学物质“读出”法被成功地应用于找寻肝素生产的一些线索,从而达到监管的目的。该质谱技术和数据分析方法应该很快能运用到其他化学和生物化学试剂检测,并适用于体积有限的样品的分析。

    详情请点击这里

     
    Screening for trace explosives by AccuTOF™-DART®: An in-depth validation study
    采用 DART-TOF 技术筛查痕量爆炸物:深度校验研究


    Ambient ionization mass spectrometry is finding increasing utility as a rapid analysis technique in a number of fields. In forensic science specifically, analysis of many types of samples, including drugs, explosives, inks, bank dye, and lotions, has been shown to be possible using these techniques . This paper focuses on one type of ambient ionization mass spectrometry, Direct Analysis in Real Time Mass Spectrometry (DART-MS or DART), and its viability as a screening tool for trace explosives analysis. In order to assess viability, a validation study was completed which focused on the analysis of trace amounts of nitro and peroxide based explosives. Topics which were studied, and are discussed, include method optimization, reproducibility, sensitivity, development of a search library, discrimination of mixtures, and blind sampling. Advantages and disadvantages of this technique over other similar screening techniques are also discussed.


    原位电离质谱在很多领域已经成为一种逐渐被广泛采用的快速分析方法。,该技术对法医学领域中很多类型的样品如药物、爆炸物、墨水、银行用染料和润肤露等成分的分析和鉴定能力尤为突出。本文主要介绍了原位电离技术中的一种:DART-MS 或 DART ,及其作为筛查手段快速分析痕量爆炸物的可行性。硝基和过氧基团的爆炸物被用来验证 DART的筛查能力,包括方法优化、重复性。灵敏度、搜库、混合物区分和盲样考核。本文同时就DART 与其他筛查技术的优缺点进行了比较与讨论。

    详情请点击这里


    Authentication of milk and milk-based foods by direct analysis in real time ionization-high resolution mass spectrometry (DART-HRMS) technique: a critical assessment
    采用实时直接分析 - 高分辨质谱(DART-HRMS)鉴定牛奶及奶制品


    The potential of direct analysis in real time (DART) ambient ionization technique coupled with high resolution mass spectrometry (HRMS) in authentication of milk and dairy products was critically assessed. In particular case, DART-HRMS was used for several scenarios: (i) discrimination among milks obtained from various farm animal species (cow, goat, and sheep), (ii) discrimination between cows´ milk produced in conventional and organic farming, and, (iii) detection of vegetable oil added to a milk-based product (soft cheese). For this purpose, a rapid profiling procedure based on examination of milk/cheese toluene extracts, was implemented. The obtained triacylglycerol (TAG) profiles (mass spectra) were processed with principal component analysis (PCA) and linear discriminant analysis (LDA). Based on LDA model, reliable differentiation of cows´ milk samples and goats'/sheep's milk was possible. The DART-HRMS procedure also allowed distinguishing milk mixtures prepared at adulteration level of 50% (v/v). The capability to recognize milk from conventional and organic farming was rather low, poor classification rates of the LDA model were obtained. On the other hand, reliable detection of the presence of vegetable oils (rapeseed, sunflower, and soybean), added to soft cheese at amount as low as 1% (w/w), was possible. Additionally, the quality of added oil in terms of degree of its oxidation could be documented.

    实时直接分析(DART)是一种原位电离技术,结合高分辨率质谱(HRMS)鉴定牛奶以及日用奶制品,是一种可靠的评估方法。在特定的条件下,通过DART-HRMS方法分析以下几种案例:区分来自不同动物种类的奶制品(奶牛、山羊、绵羊);可以辨别牛奶来自于常规生产还是来自于有机农场;可以检测奶制品中植物油(奶酪)。为了达到这个目的,建立了采用甲苯萃取牛奶/奶酪的快速分析方法。对三酰甘油(TAG)类化合物的质谱信息进行了主成分分析(PCA)和线性判别分析(LDA)。基于线性判别分析(LDA)的模型,能有效的鉴别奶牛、山羊以及绵羊生产的奶。同时,DART-HRMS 分析方法可以鉴别掺杂水平为50%(V/V)的混合牛奶。对于识别常规牛奶和来自于有机农场的牛奶的能力较差,采用线性判别分析(LDA)模型时识别率较差。另一方面,添加在奶酪中的植物油(菜籽油、向日葵油、大豆油)的检测是可靠的,尽管添加量只有1%(W/W)。甚至添加油品的氧化程度也可以藉此确定。

    详情请点击这里




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    与华质泰科同步


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    想要关注华质泰科最新动向,请关注我们的 微博LinkedIn

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    如果你愿意加入 DART 社群并与专家交流讨论 DART 技术问题,请加入我们的 DART QQ群 226596975

    If you would like to keep up with the latest developments of DART, please send us email.                             如果你愿意了解 DART 质谱新进展,请给我们留言



About IonSense


IonSense, Inc. provides OpenSpot Mass Spectrometry™ solutions to the fields of food safety, forensics, drug development, and chemical analysis. They manufacture and develop direct analysis in real time (DART®) technology licensed from JEOL USA, Inc. and atmospheric solids analysis probe (ASAP™) licensed from M&M Consulting. 



关于华质泰科
 

华质泰科生物技术(北京)有限公司(简称“华质泰科”或“ASPEC”)致力于引领行业领域中的实时科学发展潮流,通过专业的技术、成熟的产品和持续高品质的服务为客户赢得更多、更好的投资回报。华质泰科是美国 IonSense 公司 DART® 实时直接分析质谱离子源、ASAP 大气压固体分析探针、美国 Advion 公司 LESA-enabled TriVersa NanoMate 芯片多通道纳喷离子源、美国 Phoenix S&T 公司双柱-双纳喷离子源及纳升柱温盒、加拿大 Phytronix LDTD 激光热解析离子源、Tymora 公司 PolyMAC 磷酸化肽富集试剂盒、Orochem 公司手型柱及正压 SPE、Protein Metrics 公司 Byronic 糖蛋白质组学软件等技术以及产品在大中华地区的总代理商。
 

 

    

华质泰科生物技术(北京)有限公司

北京市朝阳区望京北路9号叶青大厦C座209室

 

邮编:100102     TEL:010-64399978

E-mail:info@aspectechnologies.com