Analytical strategies for controlling polysorbate-based nanomicelles in fruit juice
控制果汁中山梨醇纳米胶束的分析策略
Veronika Krtkova, Vera Schulzova, Ondrej Lacina, Vojtech Hrbek, Monika Tomaniova, Jana Hajslova ICT Prague, Technicka 3, 166 28, Prague, Czech Republic
Abstract:
Abstract: This study focused on the detection and quantification of organic micelle-type nanoparticles (NPs) with polysorbate components (polysorbate 20 and polysorbate 80) in their micelle shells that could be used to load biologically active compounds into fruit juice. Several advanced analytical techniques were applied in the stepwise method development strategy used. In the first phase, a system consisting of ultrahigh-performance liquid chromatography employing a size exclusion column coupled with an evaporative light scattering detector (UHPLC-SEC-ELSD) was used for the fractionation of micelle assemblies from other, lower molecular weight sample components. The limit of detection (LoD) of these polysorbate micelles in spiked apple juice was 500 μg mL−1. After this screening step, mass spectrometric (MS) detection was utilized to confirm the presence of polysorbates in the detected micelles. Two alternative MS techniques were tested: (i) ambient high-resolution mass spectrometry employing a direct analysis in real time ion source coupled with an Orbitrap MS analyzer (DART-Orbitrap MS) enabled fast and simple detection of the polysorbates present in the samples, with a lowest calibration level (LCL) of 1000 μg mL−1; (ii) ultrahigh-performance reversed-phase liquid chromatography coupled with high-resolution time-of-flight mass spectrometry (UHPLC-HRTOF-MS) provided highly selective and sensitive detection and quantification of polysorbates with an LCL of 0.5 μg mL−1.
摘要：
本文的研究重点是定量检测含有山梨醇成分（聚山梨醇酯-80和聚山梨醇酯-20）的有机胶束纳米粒子，这种胶束纳米粒子可负载果汁中具有生物活性的化合物成分。几种先进的分析技术根据分段发展策略被应用于检测。在第一阶段中，由 UHPLC（超高效液相色谱）、SEC（分子排阻柱）及 ELSD（蒸汽光散射检测器）组成的系统用于胶束和其他低分子量成分的分离。此方法对加入标准的苹果汁中山梨醇微胶粒的检测限（LOD）为500μg ml−1。此此筛选步骤后，质谱（MS）检测被用来进一步确定微胶粒中山梨醇的存在。我们测试了两种可供选择的质谱技术：（1）实时直接分析-高分辨率质谱系统，即 DART- Orbitrap 质谱，实现样品中山梨醇的快速简便检测；（2）UHPLC-HRTOF-MS 具有高选择性和高灵敏度的特性，能够在最低校准水平0.5μg的情况下定量山梨醇。
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