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“The Use of Chip-based nanoESI for the Analysis of Small and Large Molecules from Solution and Surfaces”
基于芯片的多通道纳升电喷雾技术用于分析溶液和表面中的生物大分子及小分子药物
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Abstract
A Chip-based nanoESI device that combines the strengths of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection, and direct surface analysis into one integrated system is introduced. The nanoESI device consists of an array of 400 nanoESI emitters with different inner diameters allowing for flow rates of 20 to 300 nL/min. The consistent nozzles are robust and bypasse any problems with non-uniformity in the spray. The spray sensing capability allows for moving simply to the next nozzle should there be an issue with spray. In applications like protein identification, non-covalent interactions, and lipids analysis, the device provides a long-lasting stable spray, and eliminates sample-to-sample carryover.
The LC/MS Fraction Collection mode gives researchers the ability to spend more time analyzing complex portions of their samples without having to reinject. It is ideally suited for researchers interested in metabolite ID, biomarker analysis, and protein characterization or any application that requires more time for analysis.
Liquid Extraction Surface Analysis (LESA) is the newest, automated, chip-based nanoESI source for mass spectrometry. It enables simple, direct nanoESI mass spectrometric analysis from a variety of surfaces, e.g. tissue slices, TLC plates, MALDI plates, and Dried Blood Spots.
SPEAKER: Mark Allen, Ph.D.
Managing Director, Europe and Asia Pacific
Advion BioSciences
Date and Time: Wed, May 18th, 2011, 10:30 – 11:30
Place: Lecture Room 1201, 2nd Floor,
Beijing Proteome Research Center
Organizer: BPRC 北京蛋白质组研究中心
Contact Miss Peng, 彭小姐
Email: bobbie_peng@139.com
Tel: (010) 8072 7777 - 1138
ASPEC Technologies 华质泰科生物技术有限公司
Contact Miss Yao, 姚小姐
Email: haiyan.yao@aspectechnologies.com
Tel: (010) 6439 9978
Cell: 15210197793
Speaker Information
Mark Allen, Ph.D. has 25 years experience in LC/MS developing new and novel ion sources and Mass Spectrometers and applying them to real world problems. He has approximately 20 publications and owes 2 patents.
1989 Ph.D. in organic mass spectrometry specializing in electrospray from King's College, London UK
1990-92 Said no to a post doc at Yale with Professor John Fenn to become a Research Scientist at Vestec Corporation, Houston Texas with Marvin Vestal to develop a new electrospray ion source that was invented during Ph.D.
1993 – 2000 Applied Biosystems®/MDS SCIEX® now ABSciex. Based in Toronto, Canada and then UK. Applications Specialist, Senior Product Manager, Director of Business Development responsible for product strategy. Introduced API III, API 300, API 3000 and QSTAR.
2001 – today Advion. Managing Director Europe and Asia Pacific. 2001 established Advion's European organization based the in UK. Current focus on developing European and Asia Pacific business for TriVersa NanoMate.
Mark Allen, Ph.D.
Mark Allen 博士是 Advion BioSeinces（Ithaca， 美国）在欧洲和亚太区的运营总监。Mark 有着25年的质谱研发和管理经历，在液质联用尤其是离子源和质谱仪的创新、制造和市场化方面有着丰富的知识和经验。
Mark 于1989年毕业于英国伦敦的 King's College，获有机质谱学博士学位。1990-92年在Vestec（休斯顿，美国）师从 Marvin Vestal 继续研发新型电喷雾离子源。1993-2000年加入Applied Biosystems / MDS SCIEX（今AB SCIEX）（多伦多，加拿大）任应用科学家、资深产品经理、业务拓展总监等，负责 API III, API3000和 QSTAR 等产品线的管理和市场化。2001至今任 Advion BioSeinces 在欧洲和亚洲的运营总监。Allen博士共发表论文20多篇，及2项专利。 |
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